Expression of CCK Receptors in Carcinoma Gallbladder and Cholelithiasis: A Pilot Study
Published: July 1, 2015 | DOI: https://doi.org/10.7860/JCDR/2015/.6152
Mohammad Shazib Faridi, Mahabir Saran Das Jaiswal, Sudhir K. Goel
1. Trainee, Department of Urology, Presently at Regional Institute of Medical Sciences, Imphal, Manipur, India.
2. Professor, Department of Surgery, Era’s Lucknow Medical College & Hospital, Lucknow, U.P, India.
3. Scientist, Petroleum Toxicology Division, Indian Institute of Toxicology Research, Lucknow, U.P, India.
Correspondence
Dr. Mohammad Shazib Faridi,
Trainee, Department of Urology, Presently at Regional Institute of Medical Sciences,
Imphal, Manipu-795004, India.
E-mail: drshazibfaridi@gmail.com
Background: Gastrin and cholecystokinin (CCK) receptors are trophic for various gastrointestinal malignancies. Their role in gallbladder cancer has not been widely studied.
Objectives: To identify expression of CCK-A and CCK-B receptors in the tissue and blood of patients suffering from carcinoma (CA) gallbladder and gallstone disease and to compare expression of CCK A and B receptors in the gall bladder tissue and blood of healthy individuals and patients of CA gallbladder, and gallstone diseases.
Materials and Methods: Forty nine subjects of both genders were recruited, comprising of 22 patients of CA gall bladder, 19 cases of cholelithiasis and, 8 normal gallbladders obtained from patients operated for trauma of the biliary system or Whipple’s procedure. RNA extraction and cDNA formation for CCK-A and CCK-B receptors were carried out. Real Time PCR was performed on cDNA and threshold cycle (Ct) value of each sample was obtained and ?Ct was calculated. Chi-square test for comparing two groups and ANOVA test for comparing multiple groups were applied and if p<0.05 then Dunnett-C test was performed.
Observation and Results: Both CCK-A and CCK-B receptors were expressed irrespective of its origin in all tissues and blood samples studied; be it normal, Cholelithiasis or CA gallbladder and there was no difference among them (p>0.05).
Conclusion: This preliminary study showed higher expression of CCK-A receptors in patients of cholelithiasis and decreased expression of CCK-A receptors in patients of CA gallbladder as compared to normal gallbladder although it did not rise to statistical significance.
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